Evaluation of intensity-based ratiometric FRET in image cytometry-Approaches and a software solution

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Quantitative intensity-based FRET approaches--a comparative snapshot.

Förster resonance energy transfer (FRET) has become an important tool for analyzing different aspects of interactions among biological macromolecules in their native environments. FRET analysis has also been successfully applied to study the spatiotemporal regulation of various cellular processes using genetically encoded FRET-based biosensors. A variety of procedures have been described for me...

متن کامل

Construction of a FRET-based ratiometric fluorescent thiol probe.

We have rationally constructed a novel FRET-based ratiometric thiol probe suitable for ratiometric imaging in living cells based on the native chemical ligation reaction.

متن کامل

A FRET-based ratiometric redox probe for detecting oxidative stress by confocal microscopy, FLIM and flow cytometry.

Understanding the role of oxidative stress in disease requires real time monitoring of redox status within a cell. We report a FRET-based, ratiometric redox probe which can be applied to monitor cellular oxidative capacity using three different modalities – confocal microscopy, fluorescence lifetime imaging and flow cytometry.

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Cytometry Part A

سال: 2009

ISSN: 1552-4922,1552-4930

DOI: 10.1002/cyto.a.20747